Fig. 4: SNAI1 knockout reduces cell invasion.
From: Loss of SNAI1 induces cellular plasticity in invasive triple-negative breast cancer cells
A, B Representative phase-contrast images from three biological repeats of MDA-MB-231-WT, SNAI1-KOs, ZR-75-1 and CS16 SNAI1-GFP#7 mammospheres embedded in collagen. Black lines demarcate the outer rim formed by invasive cells after 24 or 48 h (A) with quantification of the invasion area subtracted by the mammosphere area (B). Data are presented as mean values ± SEM of three biological replicates, each in technical triplicates and p-values are shown based on two-tailed unpaired Student’s t-test. C, D Representative images of zebrafish 24 h post-implantation with GFP-positive vasculature and CM-Dil dye-positive red cells. Extravasated cell clusters are indicated with yellow arrows (C). Quantification of extravasated cell clusters and associated significance (D). Data values from 150 WT, 142 CS16 and 152 CS19 zebrafish per cell line are presented as mean values ± SEM and p-values are shown based on two-sided Wilcoxon sum-rank U test. p-values *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
