Fig. 1: Resveratrol (RSV) counteracts ionizing radiation (IR)-induced DNA damage in keratinocytes. | Cell Death & Disease

Fig. 1: Resveratrol (RSV) counteracts ionizing radiation (IR)-induced DNA damage in keratinocytes.

From: Resveratrol rescues cutaneous radiation-induced DNA damage via a novel AMPK/SIRT7/HMGB1 regulatory axis

Fig. 1

Measurements of cell viability by CCK-8 assay (a, b) and colony formation assay (c) were performed in keratinocytes pretreated with DMSO or different concentrations of RSV with control or IR treatments. d Detection of γ-H2AX by Western blot in keratinocytes pretreated with DMSO or different concentrations of RSV with control or IR treatments. e γ-H2AX staining was performed in keratinocytes pretreated with DMSO or different concentrations of RSV with control or IR treatments. γ-H2AX positive cells were count and statistically analyzed. Scale bar: 50 μm. f Comet assay in keratinocytes pretreated with DMSO or different concentrations of RSV with control or IR treatments. Scale bar: 100 μm. Each experiment was performed in triplicates and data are presented as mean ± s.d. *p < 0.05, **p < 0.01, ***p < 0.001.

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