Fig. 3: RSV enhances the deacetylase activity of SIRT7 via AMPK-mediated SIRT7 phosphorylation. | Cell Death & Disease

Fig. 3: RSV enhances the deacetylase activity of SIRT7 via AMPK-mediated SIRT7 phosphorylation.

From: Resveratrol rescues cutaneous radiation-induced DNA damage via a novel AMPK/SIRT7/HMGB1 regulatory axis

Fig. 3

a Detection of the deacetylase activities of immunopurified SIRT7 protein from control or IR-treated keratinocytes with or without the presence of RSV (n = 3). b Detection of the deacetylase activities of in vitro-expressed SIRT7 protein with or without the presence of RSV (n = 3). c Keratinocytes were treated with RSV before IR treatment and followed by immunoprecipitation using SIRT7 antibody and detected by Western blot. d, e Keratinocytes were treated with Compound C (10 μM, 6 h) and AICAR (1 mM, 12 h) before IR treatment and followed by immunoprecipitation using SIRT7 antibody and detected by Western blot. Each experiment was performed in triplicates and data are presented as mean ± s.d. *p < 0.05, **p < 0.01, ***p < 0.001.

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