Fig. 4: SIRT7 promotes DNA damage repair by interacting with HMGB1. | Cell Death & Disease

Fig. 4: SIRT7 promotes DNA damage repair by interacting with HMGB1.

From: Resveratrol rescues cutaneous radiation-induced DNA damage via a novel AMPK/SIRT7/HMGB1 regulatory axis

Fig. 4

a Gene Ontology (GO) analysis of the proteins specifically interacting with SIRT7 identified by HPLC-MS. b, c HMGB1 was verified to interact with SIRT7 by Co-IP followed with Western blot detection. d HMGB1 was knocked down using siRNA and the relative HMGB1 expression was detected by qPCR. e γ-H2AX staining was performed in keratinocytes pretreated with siRNA targeting HMGB1 before IR treatment. Scale bar: 50 μm. f Detection of γ-H2AX and HMGB1 was detected by western blot in keratinocytes pretreated with siRNAs targeting HMGB1 before IR treatment. g Comet assay in keratinocytes pretreated with siRNAs targeting HMGB1 before IR treatment. Scale bar: 100 μm. Each experiment was performed in triplicates and data are presented as mean ± s.d. *p < 0.05, **p < 0.01, ***p < 0.001.

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