Fig. 5: SIRT7 deacetylates HMGB1 and redistributes HMGB1 to nucleus. | Cell Death & Disease

Fig. 5: SIRT7 deacetylates HMGB1 and redistributes HMGB1 to nucleus.

From: Resveratrol rescues cutaneous radiation-induced DNA damage via a novel AMPK/SIRT7/HMGB1 regulatory axis

Fig. 5

Immunoprecipitated HMGB1 acetylation was detected by Western blot in keratinocytes treated with 1.0 μM RSV and (or) siRNA targeting SIRT7 (a) or overexpression of SIRT7 (b) before IR treatment. c Immunopurified HMGB1 proteins from keratinocytes pretreated with NAM (10 mM, 6 h) were incubated with purified SIRT7 protein with or without NAD+ (5 mM). The acetylation levels were determined by immunoblotting using an anti–pan-lysine-acetylation antibody. HMGB1 and SIRT7 levels in cytoplasm and nucleus were detected by Western blot in keratinocytes after SIRT7 knockdown (d), SIRT7 overexpression (e) or RSV treatment (f). Detection of HMGB1-GFP cellular localization in keratinocytes after SIRT7 overexpression (g) or treated with RSV (h) before IR treatment. Scale bar: 50 μm. Each experiment was performed in triplicates and data are presented as mean ± s.d. *p < 0.05, **p < 0.01, ***p < 0.001.

Back to article page