Fig. 3: Roxadustat increased lipid peroxidation and calreticulin exposure.

A Representative fluorescent images of lipid peroxidation in GL261 or U87 cells after incubation with Erastin (10 μM) or RXD (100 μM towards U87, 200 μM towards GL261) with or without GSH (500 μM) for 48 h. Scale bar: 100 μm. B Representative flow cytometry analysis and statistical histogram of LPO-positive cells in GL261 or U87 cells with the different treatments. C Representative fluorescent images of CRT in GL261 or U87 cells after the different treatments. Scale bar: 100 μm. D Representative flow cytometry analysis and statistical histogram of CRT-positive cells in GL261 or U87 cells after the different treatments. *P < 0.05, **P < 0.01, ***P < 0.001.