Fig. 4: CDC6 is a downstream target of the LCAT1/IGF2BP2 complex.

A, C Volcano plots showing enrichment of IGF2BP2 target genes that were altered in IGF2BP2 knockdown (A) or LCAT1 knockdown (C) Calu1 cells. The numbers of significantly downregulated (log2 FC < −1, P < 0.05, two-sided Student’s t-test) or upregulated (log2 FC > 1, P < 0.05, two-sided Student’s t-test) genes in the IGF2BP2 target group and non-target group are displayed. Vertical dashed lines indicate the cut-off of log2 FC of 1 or –1, while the horizontal dashed line indicates the cut-off of a P value of 0.05. FC, fold change. B, D Cumulative density of mRNA log2 FC in IGF2BP2 target and non-target genes upon IGF2BP2 (B) or LCAT1 (D) knockdown. E Venn diagram of the cross-comparison of three datasets: RNA-seq of IGF2BP2 knockdown; RNA-seq of LCAT1 knockdown; and IGF2BP2 targets. F Heatmap of target genes. G, H qPCR and western blot were performed to detect CDC6 expression in IGF2BP2 knockdown and control cells. I, J qPCR and western blot were performed to detect CDC6 expression in LCAT1 knockdown and control cells. K, L qPCR and western blot were conducted to determine the impacts of LCAT1 and IGF2BP2 silencing on CDC6 expression. M Scatter plot of IGF2BP2 and CDC6 mRNA expression in lung cancers from the TCGA. N Scatter plot of LCAT1 and CDC6 mRNA expression in lung cancers from the TCGA. Student’s two-sided t-tests were used. *P < 0.05, **P < 0.01, ***P < 0.001.