Fig. 2: PERK-independent activation of p-eIF2α. | Cell Death & Disease

Fig. 2: PERK-independent activation of p-eIF2α.

From: Protein synthesis inhibition and loss of homeostatic functions in astrocytes from an Alzheimer’s disease mouse model: a role for ER-mitochondria interaction

Fig. 2

a Analysis of eIF2α phosphorylation and GADD34 expression on WT and 3Tg-iAstro. Cells were treated or not with 1 µM THG for 1 h, lysed and analysed by WB with anti- p-eIF2α, eIF2α, GADD34 and actin antibodies. Data are expressed as mean ± SEM of n = 4 (3Tg-iAstro + THG), n = 5 (3Tg-iAstro) or n = 6 (WT- and WT-iAstro + THG) independent experiments; *p < 0.05 by one-way ANOVA, Sidak’s multiple comparison; ***p < 0.001 Dunnet’s multiple comparisons. b Analysis of PERK phosphorylation and ATF4 induction on WT- and 3Tg-iAstro cells. WB analysis of cells treated as in a with anti-p-PERK, PERK, ATF4 and actin antibodies. Data are expressed as mean ± SEM from n = 3 (p-PERK and ATF) or n = 8 (PERK) independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 by one-way ANOVA, Sidak’s multiple comparison.

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