Fig. 5: Protein synthesis analysis in WT and 3xTg-AD mice. | Cell Death & Disease

Fig. 5: Protein synthesis analysis in WT and 3xTg-AD mice.

From: Protein synthesis inhibition and loss of homeostatic functions in astrocytes from an Alzheimer’s disease mouse model: a role for ER-mitochondria interaction

Fig. 5

WT and 3xTg-AD mice were i.p. injected with 225 mg/Kg of puromycin and sacrificed 1.5 h post-injection. a WB with anti-puromycin, anti-actin antibodies, and ponceau staining on hippocampal homogenates. Data are expressed as mean ± SEM of 13 independent WB from two mice per genotype. **p < 0.01, unpaired t-test. b IF with anti-puromycin antibody (green) and DAPI (blue) of hippocampal brain slices. Data are expressed as mean ± SEM of four sections collected from two mice per genotype. Scale bar = 50 μm.

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