Fig. 5: Impaired survival of 4xSNCA mDA neurons exposed to fibrils. | Cell Death & Disease

Fig. 5: Impaired survival of 4xSNCA mDA neurons exposed to fibrils.

From: Modeling native and seeded Synuclein aggregation and related cellular dysfunctions in dopaminergic neurons derived by a new set of isogenic iPSC lines with SNCA multiplications

Fig. 5

A Schematic view of the protocol to intoxicate cortical and DA neuronal cultures with P91 αSyn fibrils. B Live/dead fluorescence staining and quantification of iPSC-derived cortical neurons with or without exposure to P91 fibrils. C Live/dead fluorescence staining in iPSC-derived DA neurons with or without exposure to P91 fibrils and relative quantification (for each group n = 50 fields, automatic counts). Values are mean ± SEM of n = 3 biologically independent experiments. **p < 0.01, ***p < 0.001. Statistical analysis is performed using two-way ANOVA followed by the Bonferroni post-test. Scale bars, 100 µm.

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