Fig. 1: Glutamine deprivation induces apoptosis in tumor cell lines of different origins by a mitochondria-operated pathway. | Cell Death & Disease

Fig. 1: Glutamine deprivation induces apoptosis in tumor cell lines of different origins by a mitochondria-operated pathway.

From: Limiting glutamine utilization activates a GCN2/TRAIL-R2/Caspase-8 apoptotic pathway in glutamine-addicted tumor cells

Fig. 1

A HCT116 (left) or MDA-MB468 (right) cells were cultured for 48 h in medium with or without glutamine (Gln) in the presence or absence of Q-VD-OPh (20 µmol/L). Apoptosis was determined as described in Materials and Methods section. B Apoptosis was assessed in HCT116 WT or Bax/Bak KO cells cultured in the presence or absence of glutamine for 48 h (left panel). Procaspase-3 (proC3) and cleaved caspase-3 (cC3) levels were assessed by Western blotting in HCT116 WT or Bax/Bak KO cells starved of glutamine for 30 h (right panel). GAPDH was used as protein-loading control. C pBabe or Bcl-xL-overexpressing MDA-MB468 cells were cultured for 48 h in medium with or without glutamine and apoptosis assessed as described in A. Bcl-xL overexpression was confirmed by Western blotting. Data are presented as mean ± SD from at least three independent experiments. *P < 0.05; ***P < 0.001; ****P < 0.0001; two-way ANOVA test. Tukey’s multiple comparison test.

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