Fig. 2: The amino-terminal region of CD95 is lost in all cancer cells and normal blood cells but macrophages.
From: MMP7 cleavage of amino-terminal CD95 death receptor switches signaling toward non-apoptotic pathways
A Indicated cancer cell lines were stained with DX2 and N18 as primary antibodies (red lines) or with isotype-matched control IgG (shadowed histogram) and CD95 expression was evaluated by flow cytometry. B Normal human peripheral blood cells (PBLs) were stained by DX2 or N18 (red lines) or isotype-matched control IgG (shadowed histogram) and CD95 expression was analyzed by flow cytometry. Cell populations were evaluated by using specific markers (T cells (CD3+), B cells (CD19+); NK cells (CD56+), NKT cells (CD3+CD56+), monocytes (CD14+SIGLEC5+) and neutrophils (CD14−SIGLEC5+)). Note that the N-terminal domain of CD95 was only detected in the monocyte population.
