Fig. 4: Long-term inhibition of EGFR kinase leads to decreased decay of EGFL7 transcripts. | Cell Death & Disease

Fig. 4: Long-term inhibition of EGFR kinase leads to decreased decay of EGFL7 transcripts.

From: EGFL7 drives the evolution of resistance to EGFR inhibitors in lung cancer by activating NOTCH signaling

Fig. 4

A, B Schematic of EGFL7 transcripts and open reading frames (ORF) in certain transcripts. C Two small alignment regions corresponding to two translation initiation sites (TISs) of EGFL7 in ten species. The evolutionary tree obtained from NCBI Taxonomy database is presented on the left. D The electrophoresis presenting specific EGFL7 transcript in PC9 cells treated with osimertinib for the indicated time points. E Western blot analysis showing the levels of EGFR, pEGFR, RNET1, RNET2, RNET3 and EGFL7 in response to the indicated EGFR kinase inhibitors. F Western blot analysis showing the level of EGFR, pEGFR, RNET3 and EGFL7 in PC9 cells treated with osimertinib for the indicated time points. GAPDH was used as a loading control. G Schematic of declined decay of EGFL7 transcripts in response to EGFR kinase inhibition.

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