Fig. 1: GPER expression is decreased in leukemic cells. | Cell Death & Disease

Fig. 1: GPER expression is decreased in leukemic cells.

From: Targeted activation of GPER enhances the efficacy of venetoclax by boosting leukemic pyroptosis and CD8+ T cell immune function in acute myeloid leukemia

Fig. 1: GPER expression is decreased in leukemic cells.

A, B GPER levels in AML patients and HD were identified from Oncomine and Beat AML databases. C–E qRT-PCR, western blot and IHC staining analyses of GPER levels in primary AML blasts and counterparts from HD (Scale bar: 10 μm). F, G qRT-PCR and western blot analyses of GPER levels in AML cell lines. H The subcellular localization of GPER (green) was detected by IF staining. The nucleus was stained blue with DAPI (Scale bar: 25 μm). I Potential methylation sites in GPER CpG island were identified using MethPrimer software. J Methylation status of GPER promoter was validated by Bisulfite genomic DNA sequencing. Each black dots represented a methylated cytosine residue in the CpG islands whereas each white dots represented unmethylated CpG dinucleotides. K qRT-PCR analysis of GPER level in the AML cells treated with 1 μM 5-Aza for 48 h. The data are expressed as the mean ± SD (n = 3). **p < 0.01. ns, not significant.

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