Fig. 1: The exogenous addition of 27HC depletes hematopoietic stem and progenitor cells (HSPCs).

BM cells are treated with 13 µM Cholesterol or 6.2 µM 27OHChol for 48 h, respectively. A Study overview. B FACS plot showing the frequency of LK, LKS, HPC, and HSC populations after 27HC treatment. C The frequencies of Lin⁻Sca1⁻cKit⁺ cells (LK), Lin⁻Sca1⁺cKit⁺ cells (LKS), HPCs (Lin⁻Sca1⁺cKit⁺ CD48⁺), and HSCs (Lin⁻Sca1⁺cKit⁺CD150⁺CD48^-, SLAM cells) were decreased in the BM cells after exogenous 27HC treatment. D FACS plot showing the frequency of CMP, GMP, MEP, and CLP populations after 27HC treatment. E The frequencies of common myeloid progenitor (CMP) cells (Lin⁻Sca1⁻cKit⁺CD34⁺CD16/32^-), granulocyte-macrophage progenitor (GMP) cells (Lin⁻Sca1⁻cKit⁺CD34⁺CD16/32⁺), megakaryocyte-erythroid progenitor (MEP) cells (Lin⁻Sca1⁻cKit⁺CD34⁻CD16/32⁻), and common lymphoid progenitor (CLP) cells (Lin⁻ Sca1^lowcKit^low CD127⁺) were decreased in the BM cells after exogenous 27HC treatment. F FACS plot showing the frequency of monocytes, neutrophils, B cells, and T cells after 27HC treatment. G The frequencies of monocytes (CD11b⁺ cells), neutrophils (CD11b⁺Gr1⁺ cells), B cells (B220⁺ cells) and T cells (CD3⁺ cells) were observed after 27HC treatment. Data are presented as mean ± SEM. (*p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001 vs. control; ^#p ≤ 0.05, ^##p ≤ 0.01, and ^###p ≤ 0.001 vs. Chol). (n = 2 independent experiments and 3 total measurements per treatment).