Fig. 4: Exogenous addition of 27HC affects leukemic cell growth in vitro and in vivo. | Cell Death & Disease

Fig. 4: Exogenous addition of 27HC affects leukemic cell growth in vitro and in vivo.

From: Role of reactive oxygen species in regulating 27-hydroxycholesterol-induced apoptosis of hematopoietic progenitor cells and myeloid cell lines

Fig. 4

HL60, KG1a, and K562 myeloid leukemic cells are treated with 13 µM Cholesterol or 6.2 µM 27OHChol for 48 h, respectively. A Study overview. B HL60, KG1a, and K562 myeloid leukemic cells were decreased after 27HC treatment. C The exogenous 27HC-treated HL60, KG1a, and K562 myeloid leukemic cells showed a greater percentage of apoptotic cells than did control cells. D The exogenous 27HC-treated HL60, KG1a, and K562 myeloid leukemic cells showed greater ROS production than control cells. E 27HC increases the activity of the ER stress response in HL60 and K562 cells. Data are presented as mean ± SEM. (**p ≤ 0.01 and ***p ≤ 0.001 vs. control; ##p ≤ 0.01 and ###p ≤ 0.001 vs. Chol). (n = 2 independent experiments and 3 total measurements per treatment).

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