Fig. 5: scRNA-seq analysis of acute myeloid leukemia (AML) dataset GSE116256.

A Survival curve relative to CYP7B1 expression (high expression (n = 56) and low expression (n = 55)) in individuals affected by acute myeloid leukemia (AML) and based on GSE12417 (log-rank test, p = 0.00982) (https://easysurv.net/#/app/home). B tSNE plot of AML patients and healthy subjects. Cells are annotated into six cell types. Granulocyte-macrophage progenitor (GMP); progenitor (Prog); promonocyte (ProMono); hematopoietic stem cell (HSC); conventional dendritic cell (cDC); monocyte (Mono). The cells from the healthy group and AML patients are located below. C The stacked bar plot shows the composition of cell types. Top bar plots indicate the proportion of cell types in 4430 cells from healthy donors and 17,015 cells from AML patients. In the middle, the plot exhibits components and proportions of normal cells and malignant cells in healthy donors and AML patients. At the bottom, it shows the proportion of cell types in 7956 normal cells and 13489 malignant cells. D Gene expression of CYP7B1. From left to right, plots describe expression levels of CYP7B1 in whole-cell types, HSC, progenitor, and cDC. Expression levels are calculated as log-normalized counts and the Wilcox rank-sum test was performed to compare normal cells and malignant cells. The red dot in the plot represents the mean expression value. E Gene Ontology terms of CYP7B1-correlated genes in HSC. The top 10 biological processes of genes that are differentially correlated to CYP7B1 between malignant and normal cells are listed. The color of the dot represents the significance of the term and size refers to the percentage of enriched genes. F Gene Ontology analyses are similar to (E), but for cDC.