Fig. 7: Downregulation of SOCS5 inhibits PM-induced HCC metastasis. | Cell Death & Disease

Fig. 7: Downregulation of SOCS5 inhibits PM-induced HCC metastasis.

From: SOCS5 knockdown suppresses metastasis of hepatocellular carcinoma by ameliorating HIF-1α-dependent mitochondrial damage

Fig. 7

A Schematic diagram of the protocol used to measure the changes in the expression of hypoxic and EMT markers following transient left hepatic artery and vein ligation (LHAVL) of the left lobe of the liver. B Representative images of orthotopic HCC model (LV-SOCS5). C Western blot analysis of SOCS5, HIF-1α, and E-Cadherin protein expression in orthotopic HCC treated with LHAVL for 2 h. D Schematic diagram of the protocol used to measure the changes in the expression of hypoxic and EMT markers following intermittent LHAVL of the left lobe of the liver. E Representative images of orthotopic HCC model (shSOCS5) treated with intermittent LHAVL. F Representative images of the corresponding lung metastasis model of orthotopic HCC after intermittent LHAVL. The number of metastatic nodules in the lungs. G Immunohistochemical staining of SOCS5, HIF-1α, and EMT protein expression in the liver and lungs. *P < 0.05, **P < 0.01, and ***P < 0.001.

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