Fig. 4: Effect of HSPB8 overexpression on NRAS pathway in A375 cells. | Cell Death & Disease

Fig. 4: Effect of HSPB8 overexpression on NRAS pathway in A375 cells.

From: HSPB8 counteracts tumor activity of BRAF- and NRAS-mutant melanoma cells by modulation of RAS-prenylation and autophagy

Fig. 4

a WB analysis of RAS and BRAFV600E protein levels in A375 cells overexpressing HSPB8 (48 h). Tubulin was used as loading control. b WB analysis of RAS protein levels in plasma membranes (Mb) of BLM cells expressing HSPB8 (48 h) compared with total protein extracts (EP). c Effect of HSPB8 overexpression (48 h) on MAPK pathway. Tubulin was used as loading control. Three independent biological samples for each condition were analyzed (N = 3), bar graph represents the mean optical density ± SD. Statistical analysis was performed using One-way ANOVA. d Effect of HSPB8 overexpression (48 h) on Akt/mTOR pathway. Tubulin was used as loading control. Three independent biological samples for each condition were analyzed (N = 3), bar graph represents the mean optical density ± SD. Statistical analysis was performed using One-way ANOVA followed by Bonferroni post-hoc test (**p < 0.01 vs. C and mock ***p < 0.001 vs. C and mock). e Schematic representation of HSPB8 effect on NRASwt and BRAFmut activity.

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