Fig. 6: Effect of HSPB8 overexpression on autophagy in BLM and A375 cells. | Cell Death & Disease

Fig. 6: Effect of HSPB8 overexpression on autophagy in BLM and A375 cells.

From: HSPB8 counteracts tumor activity of BRAF- and NRAS-mutant melanoma cells by modulation of RAS-prenylation and autophagy

Fig. 6

a, b WB analysis of LC3 in BLM (a) and A375 (b) overexpressing HSPB8 (48 h). c LC3 puncta (green) and HSPB8 expression (red) were carried out by immunofluorescence in BLM and A375 cells overexpressing HSPB8 (48 h). Nuclei were stained by Hoechst. Scale bar, 20 μm. d, f Protein expression of p62 in BLM (d) and A375 (f) cells overexpressing HSPB8 (48 h). The quantification results were calculated over three individual experiments. Bar graph represents the mean optical density ± SD. Statistical analysis was performed using One-way ANOVA followed by Bonferroni post-hoc test (**p < 0.01 vs. Control (C) and mock; ***p < 0.001 vs. Control (C) and mock). e, g p62 mRNA levels in BLM (e) and A375 (g) cells overexpressing HSPB8 (24 h). Data have been normalized to the amount of RPLP0 mRNA. Bar graph represents the mean of three independent biological samples (N = 3) ±SD. Statistical analysis was performed using Student’s t-test (*p < 0.05 vs. mock). h, i WB analysis of LC3 and p62 in BLM (h) and A375 (i) cells overexpressing HSPB8 (48 h) treated with CQ 10 μM for the last 24 h of transfection.

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