Fig. 2: SLC1A5_var ameliorates LPS-induced dysfunction of mitochondrial in astrocytes. | Cell Death & Disease

Fig. 2: SLC1A5_var ameliorates LPS-induced dysfunction of mitochondrial in astrocytes.

From: Mitochondrial glutamine transporter SLC1A5_var, a potential target to suppress astrocyte reactivity in Parkinson’s Disease

Fig. 2

Astrocytes were transfected with SLC1A5_var for 48 h and stimulated with LPS (100 ng/ml) for 6 h. Effect of SLC1A5_var overexpression (A) (n = 6 from 6 mice/group) or knockdown (D) (n = 4 from 4 mice/group) on the oxygen consumption rate in astrocytes stimulated with LPS. Effect of SLC1A5_var overexpression (B) or knockdown (E) on basal respiration of astrocytes. Effect of SLC1A5_var overexpression (C) or knockdown (F) on maximum respiration from oxidative phosphorylation in astrocytes. The non-mitochondrial respiration was not subtracted for the quantification. Each data point represents a separate well of the seahorse plate, and the astrocytes in each well were isolated from different mice. *P < 0.05, **P < 0.01 and ***P < 0.001 vs control, ##P < 0.01 and ###P < 0.001 vs LPS group, two-way ANOVA followed by Tukey’s post hoc test. G The morphology of mitochondria is observed in transmission electron microscopic (TEM). Blue arrows: mitochondria. Scale bar, 1 μm (left), 500 nm (Enlarged vision).

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