Fig. 6: SLC1A5_var inhibited LPS-induced astrocytic inflammatory cytokines and down-regulates TLR4 signal pathway. | Cell Death & Disease

Fig. 6: SLC1A5_var inhibited LPS-induced astrocytic inflammatory cytokines and down-regulates TLR4 signal pathway.

From: Mitochondrial glutamine transporter SLC1A5_var, a potential target to suppress astrocyte reactivity in Parkinson’s Disease

Fig. 6

Astrocytes were transfected with SLC1A5_var (OE) or with SLC1A5_var siRNA for 48 h and stimulated with LPS (100 ng/ml) for 6 h. The mRNA expression levels of Tnf-α (A), Il-6 (B), Il-1β (C), and Il-18 (D) stimulated with LPS were detected by qRT-qPCR(n = 3). Secretion of TNF-α (E), IL-6 (F), IL-1β (G) and IL-18 (H) in astrocytes stimulated with LPS was detected by ELISA (n = 3). The protein level of TLR4, p-p38, p-JNK and p-AKT was determined by western blot (C, F) while SLC1A5_var was overexpression (I) or knockdown (N) and quantified by densitometry (J–M and O–R) (n = 3). Data are shown as the mean ± S.E.M. **P < 0.01, ***P < 0.001 vs control, ns, P > 0.05, #P < 0.05, ##P < 0.01 and ###P < 0.001 vs LPS group, two-way ANOVA followed by Tukey’s post hoc test.

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