Fig. 3: Mybpc3 deficiency enhanced myofibroblasts conversion.
From: MYBPC3 deficiency in cardiac fibroblasts drives their activation and contributes to fibrosis
A–C The transcript (A) and protein (B, C) expression of COL1A1, α-SMA, and TGF-β1 in WT and MYBPC3-KO fibroblasts. ****P < 0.0001. D–F The deficiency of Mybpc3 gene increased fibroblast proliferation and reduced apoptosis. *P < 0.05; **P < 0.01; ***P < 0.001. G Cell cycle analysis showed that Mybpc3 disruption increased cells in the G2/M phase while slightly reducing cells in the G0/G1 phase. ns, not significant; **P < 0.01. H Measurement of fibroblast migration by the cell wound scratch assay. ****P < 0.0001. I, J Measurement of TGF-β1 in the culture supernatant and cell lysate of WT and MYBPC3-KO fibroblasts. **P < 0.01.
