Fig. 4: NALT1 promotes PEG10 expression in CRC.
From: LncRNA NALT1 promotes colorectal cancer progression via targeting PEG10 by sponging microRNA-574-5p
A Heatmap revealing the significantly differentially expressed mRNA in NALT1-knockdown and NC HCT116 cells. Green and red denote downregulated and upregulated mRNAs, respectively. B Genes were negatively and positively regulated by NALT1, respectively (fold-change>1.5, q < 0.05). C Ten top genes regulated by NALT1 with fold-change>1.5 and p<0.05 are drawn in the volcano plot. D–G RT-qPCR and WB analyses of PEG10 expression after knockdown or overexpression of NALT1 in HCT116 cells. H Gene expression correlation between NALT1 (x) and PEG10 (y) in HCT116 cells (R = 0.2106, p = 0.0317). I CCK8 assay was applied to measure the cell proliferation in PEG10-knockdown or overexpression HCT116 cells. K WB analyses the efficiency of PEG10 eukaryotic expression plasmid in HCT116 cells. J Transwell assays were applied to measure the migratory and invasive capability of HCT116 cells with PEG10 knockdown or overexpression. L CCK8 assay was applied to measure the cell proliferation in NALT1-knockdown or NC HCT116 cells after transfection with PEG10 overexpression plasmid or control.
