Fig. 5: NALT1 serves as a sponge of miRNA-574-5p.
From: LncRNA NALT1 promotes colorectal cancer progression via targeting PEG10 by sponging microRNA-574-5p
A, B Subcellular localization analysis of NALT1 in HCT116 cells. Blue, DAPI; Red, NALT1. C Two miRNAs targeted by NALT1 were estimated by cross-analysis with TargetScan, Starbase, and miRanda databases. D The efficiency of the biotinylated NALT1 probe in HCT116 cells was verified by RT-qPCR. E The relative expression of miRNA-574-5p enriched by the NALT1 probe in HCT116 cells was evaluated by RT-qPCR. F Schematic diagram of Mut-NALT1 and WT-NALT1 LR vectors. G HCT116 was cotransfected with miRNA-574-5p mimic and Mut or WT NALT1 LR vectors, and the activities of the LR vectors were detected. H, I RT-qPCR and CCK8 assays were conducted to measure the cell proliferation in NALT1-knockdown or NC HCT116 cells after transfection with miRNA-574-5p inhibitor or NC. J Transwell assays were conducted to measure the migration and invasion capability in NALT1-knockdown or NC HCT116 cells after transfection with miRNA-574-5p inhibitor or NC. K, L RT-qPCR and CCK8 assays were performed to measure the NALT1 level and cell proliferation in NALT1-overexpression or NC HCT116 cells after transfection with miRNA-574-5p mimic or NC. M Transwell assays were conducted to measure the migration and invasion capability in NALT1-overexpression or NC HCT116 cells after transfection with miRNA-574-5p mimics or NC.
