Fig. 4: Peptains inhibit glial activation in MB-induced ocular hypertension in mice. | Cell Death & Disease

Fig. 4: Peptains inhibit glial activation in MB-induced ocular hypertension in mice.

From: Peptains block retinal ganglion cell death in animal models of ocular hypertension: implications for neuroprotection in glaucoma

Fig. 4

The induction of ocular hypertension was performed as described in Fig. 3. MB was injected into the anterior chamber, and after 3 weeks, peptain-1 or peptain-3a was injected intravitreally at 1 µg/µl in PBS (with 0.1% DMSO) and subsequently once a week for 3 weeks. Six weeks after MB injection, retinal flatmounts were immunostained for Iba1 (A, B) and GFAP (C, D) to identify activated microglia and astrocytes. The bar graph B shows the fold change (mean ± SD) of Iba1 positive cells and D shows the fold change (mean ± SD) of GFAP fluorescence intensity in the ganglion cell layer. Each data point represents one animal. Scale bar = 100 μm.

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