Fig. 5: Protective effects of peptains against RGC death in SO-induced ocular hypertension. | Cell Death & Disease

Fig. 5: Protective effects of peptains against RGC death in SO-induced ocular hypertension.

From: Peptains block retinal ganglion cell death in animal models of ocular hypertension: implications for neuroprotection in glaucoma

Fig. 5

A The SO and peptain injection timeline is shown. Mice were injected with silicone oil (SO, 2 µl, 1000 mPa.s) into the anterior chamber until the oil covered the iris; after 2 weeks, the oil was removed. Peptain-1 or peptain-3a was intravitreally injected at 1 µg in 1 µl of PBS (with 0.1% DMSO) 2 days after SO removal. B The IOP was monitored once a week during the 28-day follow-up period. The injection of SO elevated the IOP, and its removal returned IOP to the normal range. C Mice were euthanized, and the retinal flatmounts were immunostained with Brn3a at 2 weeks post-SO treatment, at 2 weeks post-SO treatment + 2 weeks post-SO removal, (2 + 2 weeks), and as another control, we injected SO, and mice were euthanized after 4 weeks without removal (4 weeks). The bar graph shows the quantification of Brn3a-positive RGCs in retinal flatmounts. N = 4. D Two weeks after SO removal (2 + 2 weeks), retinal flatmounts were immunostained for Brn3a (green) and βIII-tubulin (red) antibodies. Confocal microscopic images were captured from the mid-peripheral retina. Scale bar = 100 μm. E The bar graph shows the quantification of Brn3a+ RGCs in retinal flatmounts. Each data point represents one animal. F Confocal images showed the anterograde transportation of CT-B along the entire length of the optic nerve to the chiasm. G The CT-B intensity was quantified in the proximal, intermediate, and distal regions of the optic nerve. Scale bar = 1 mm. Data represent the mean ± SD of 4–6 independent experiments. *p < 0.05, **p < 0.01, ****p < 0.0001. ns = not significant. Control = contralateral uninjured eye.

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