Fig. 6: Peptains inhibit glial activation in SO-induced ocular hypertension. | Cell Death & Disease

Fig. 6: Peptains inhibit glial activation in SO-induced ocular hypertension.

From: Peptains block retinal ganglion cell death in animal models of ocular hypertension: implications for neuroprotection in glaucoma

Fig. 6

The induction of SO-mediated ocular hypertension was performed as described in Fig. 5. SO was injected into the anterior chamber, and after 2 weeks, the oil was removed. Peptain-1 or peptain-3a was intravitreally injected at 1 µg in 1 µl of PBS (with 0.1% DMSO) 2 days after SO removal. Four weeks after injury, retinal flatmounts were immunostained with Iba1 (A, B) and GFAP (C, D) antibodies to identify activated microglia and astrocytes. The bar graph B shows the fold change (mean ± SD) of Iba1 positive cells and D shows the fold change (mean ± SD) of GFAP fluorescence intensity in the ganglion cell layer. Each data point represents one animal. Scale bar = 100 μm.

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