Fig. 1: Subcellular localization of RAI1 in SMS patient-derived cells. | Cell Death & Disease

Fig. 1: Subcellular localization of RAI1 in SMS patient-derived cells.

From: Retinoic acid-induced 1 gene haploinsufficiency alters lipid metabolism and causes autophagy defects in Smith-Magenis syndrome

Fig. 1: Subcellular localization of RAI1 in SMS patient-derived cells.The alternative text for this image may have been generated using AI.

A The RT–PCR analysis of the transcript levels of RAI1 revealed reduced gene expression in SMS cells compared with control cells (n = 6–7 biological replicates). B An immunofluorescence analysis of the subcellular localization of RAI1 in SMS and control cells revealed that wild-type and mutant RAI1 localize to the nucleus and cytosol. RAI1 (green) was detected using a specific antibody, and nuclei were detected with Hoechst (blue) (n = 3 biological replicates). Scale bar = 25 µm. Representative images are shown. C Densitometry showing the RAI1 levels (n = 15 cells from 5 randomly selected fields from 3 independent experiments of each cell line). Graphs: mean ± SEM, one-way ANOVA + Newman–Keuls post hoc test, *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant.

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