Fig. 4: Accumulation of lipid droplets in SMS patient-derived cells. | Cell Death & Disease

Fig. 4: Accumulation of lipid droplets in SMS patient-derived cells.

From: Retinoic acid-induced 1 gene haploinsufficiency alters lipid metabolism and causes autophagy defects in Smith-Magenis syndrome

Fig. 4: Accumulation of lipid droplets in SMS patient-derived cells.The alternative text for this image may have been generated using AI.

A Left panel: Representative fluorescence microscopy images of lipid droplets (LDs) colored with Oil Red O in SMS and CTR cells (n = 5 biological replicates). Middle panel: Quantification of the optical density of nine independent wells from five biological replicates. Right panel: Quantification of the number of LDs per cell among 150 cells from three biological replicates. B, C BODIPY staining analysis of the number and size of LDs in SMS and CTR cells. Left panel: Representative images of 10 fields from three biological replicates. Middle panel: Quantification of the LD number of 10 cells from 10 randomly selected fields from three independent experiments of each cell line. Right panel: Quantification of the size of LDs per cell among 10 cells from 10 randomly selected fields from three independent experiments of each cell line. D Oil Red O staining in four SMS and four CTR cell lines treated with vehicle and oleic acid (OA, 50 μM, 48 h). Left panel: Representative images. Right panel: Quantification of the number of LDs per cell among 150 cells from three biological replicates. Graphs: mean ± SEM, one-way ANOVA + Newman–Keuls post hoc test, *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant.

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