Fig. 3: Overexpression of linc00976 promotes proliferation and metastasis and inhibits ferroptosis of CCA cells. | Cell Death & Disease

Fig. 3: Overexpression of linc00976 promotes proliferation and metastasis and inhibits ferroptosis of CCA cells.

From: JUND/linc00976 promotes cholangiocarcinoma progression and metastasis, inhibits ferroptosis by regulating the miR-3202/GPX4 axis

Fig. 3

a–d CCK8, EdU (scale bar, 50 μm), generation of CCA spheroids, and colony formation assays detected the effect of linc00976 overexpression on cell proliferation ability. e–i Effects of linc00976 overexpression on ROS, GSH, MDA, iron progression, and GPX activities in CCA cells. j Western blotting detected the effect of linc00976 overexpression on the expression of ferroptosis-associated biomarkers. k Transwell migration and Matrigel invasion assays detected the effect of linc00976 overexpression on cell migration and invasion. Scale bar, 50 μm. l Western blotting determined the effect of linc00976 overexpression on the expression of EMT-associated biomarkers. m, n Effect of linc00976 overexpression on tumor growth and tumor weight in nude mice. o Representative images of xenograft tumors in nude mice after subcutaneous implantation of HuCCT1 cells stably infected with linc00976-overexpression lentivirus or control lentivirus. p Tumor tissues were sectioned and stained for KI67 and PCNA by IHC. Scale bar, 100 μm. q, r Representative images of lung tissues and lung sections stained with H&E in the lung metastatic mice model. Scale bar, 500 μm. *P < 0.05, **P < 0.01. CCA cholangiocarcinoma, EMT epithelial–mesenchymal transition, GPx glutathione peroxidase, GSH glutathione, H&E hematoxylin and eosin, IHC immunohistochemistry, MDA malondialdehyde, ROS reactive oxygen species.

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