Fig. 2: Exosomes transport miR-197-3p from LUAD cells to HUVECs in vitro. | Cell Death & Disease

Fig. 2: Exosomes transport miR-197-3p from LUAD cells to HUVECs in vitro.

From: Cancer-derived exosomal miR-197-3p confers angiogenesis via targeting TIMP2/3 in lung adenocarcinoma metastasis

Fig. 2

A The miR-197-3p level in NLEC, LUAD cells and HUVECs was detected by qRT-PCR. B The morphologies of A549 or H1299 cells-derived exosomes were observed by TEM. C Exosome markers and negative control GM130 were detected by western blot. D The uptake of PKH26-labeled exosomes was examined by confocal microscopy. Red: PKH26; Blue: DAPI. E Cellular and exosomal miR-197-3p levels in A549 and H1299 cells were detected by qRT-PCR. F HUVECs were treated with A549 or H1299 cells-derived exosomes for 0, 6, 12 and 24 h. MiR-197-3p level in HUVECs was detected by qRT-PCR. Data were representative images or were expressed as the mean ± SD of n = 3 experiments. *P < 0.05, **P < 0.01, ***P < 0.001.

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