Fig. 6: Gpr68 inhibition alleviates the expression of inflammatory cytokines and Mmp9 in Tsc2 deficient macrophages.
From: Loss of myeloid Tsc2 predisposes to angiotensin II-induced aortic aneurysm formation in mice
Macrophages from AngII-infused Tsc2MKOApoE–/– mice were treated with shRNA-Gpr68 or negative control for 24 h. A Quantitative real-time PCR analysis of IL-6, IL1-β, MCP-1, and Mmp9 mRNA expression (n = 5). B, C Western blot analysis of Mmp9 and Gpr68 protein expression (n = 4). D Gelatin zymography analysis of Mmp9 activity (n = 4). E Western blot analysis of phosphorylated CREB expression (n = 4). *P < 0.05, vs. macrophages treated with negative control or macrophages from AngII-infused Tsc2WTApoE–/– mice. F Chromatin immunoprecipitation-seq assay of the occupancy of Mmp9 and TSP-1 gene promoters by CREB (data from GSM2663863 dataset).
