Fig. 2: COX7A1 enhances the sensitivity of NSCLC cells to cysteine deprivation-induced ferroptosis. | Cell Death & Disease

Fig. 2: COX7A1 enhances the sensitivity of NSCLC cells to cysteine deprivation-induced ferroptosis.

From: COX7A1 enhances the sensitivity of human NSCLC cells to cystine deprivation-induced ferroptosis via regulating mitochondrial metabolism

Fig. 2

The ferroptosis model in NSCLC cells was induced by cellular cysteine (CC) starvation and the treatment of erastin (5 μM) for 24 h respectively. Then, cell viability was evaluated using CCK-8 method (A). To analyze the ferroptosis level, MDA (B) and 4-HNE (C) levels were measured in different groups. The expression of PTGS2 (D) and lipid peroxidation (E) were detected using qPCR and BODIPY staining respectively. Scale bar = 10 μm. Results are expressed as mean ± SD, and the P value less than 0.05 was considered statistically significant. *:P < 0.05 compared with the Ctrl group in CC(+)Erastin(−) condition. #:P < 0.05 compared between Ctrl group and Overexpression (OE) group in same condition. &:P < 0.05 compared with the OE group in CC(+)Erastin(−) condition.

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