Fig. 1: YY1 enhances METTL3 expression as a transcription factor. | Cell Death & Disease

Fig. 1: YY1 enhances METTL3 expression as a transcription factor.

From: HDAC1/3-dependent moderate liquid–liquid phase separation of YY1 promotes METTL3 expression and AML cell proliferation

Fig. 1

A Pearson correlation of YY1 with METTL3 in expression in AML samples from TCGA. B Pearson correlation of YY1 with METTL3 in expression in AML samples from Qilu Hospital of Shandong University as detected by qPCR. n = 78. C qPCR analysis of the expression of YY1 and METTL3 in THP-1 or Kasumi-1 cells transfected with LV5-homo-YY1 or LV5-NC. *P < 0.05; **P < 0.01; ****P < 0.0001, t-test. D qPCR analysis of the expression of YY1 and METTL3 in THP-1 or Kasumi-1 cells transfected with LV3-shYY1-1 or LV3-shYY1-2 or LV3-SRC. *P < 0.05; **P < 0.01; ***P < 0.001, t-test. E, F Western blotting analysis of the expression of YY1 and METTL3 in THP-1 cells (E) or Kasumi-1 cells (F) that overexpress or interfere YY1. *P < 0.05; **P < 0.01; ***P < 0.001, t-test. G The conservative binding motif of YY1 to the METTL3 promoter predicted by the JASPAR website. H Binding sites of YY1 to the METTL3 promoter region. I CHIP-qPCR experiments in THP-1 cells. **P < 0.01; ***P < 0.001; NS = nonsignificant, t-test. J Luciferase activity in HEK293T cells co-transfected with the YY1 overexpression plasmids and the luciferase reporter vectors assessed by luciferase reporter assay. *P < 0.05; **P < 0.01; ***P < 0.001; NS = nonsignificant, t-test. H Luciferase activity in HEK293T cells co-transfected with the YY1 siRNA and the luciferase reporter vectors assessed by luciferase reporter assay. *P < 0.05; NS = nonsignificant, t-test.

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