Fig. 6: YY1 HDAC1/3 binding sites mutations affects its regulation of METTL3 and AML cell proliferation. | Cell Death & Disease

Fig. 6: YY1 HDAC1/3 binding sites mutations affects its regulation of METTL3 and AML cell proliferation.

From: HDAC1/3-dependent moderate liquid–liquid phase separation of YY1 promotes METTL3 expression and AML cell proliferation

Fig. 6

A Domain structure of the YY1 protein. YY1-6R represent YY1 arginine mutant. B Coimmunoprecipitation (Co-IP) assays for association of HDAC1/3-HA with YY1-6R-EGFP. Co-transfected YY1-EGFP or YY1-6R-EGFP with HDAC1/3-HA, the cell extracts were prepared and precipitated with anti-EGFP beads, and detected by using immunoblotting with anti-EGFP and anti-HA antibodies (n = 3). C Luciferase activity in 293T cells co-transfected with the wide type YY1 or Flag-YY1-6R and the luciferase reporter vectors, with or without CHI treatment for 24 h, assessed by luciferase reporter assay. **P < 0.01; ***P < 0.001; NS = nonsignificant, t-test. D THP-1 cells were transfected with Flag-YY1 or Flag-YY1-6R mutant plasmids, with or without 4 μM CHI treatment for 1 h, and performed immunofluorescence analysis with anti-Flag antibody. E The number of fluorescent particles of figure (D). ****P < 0.0001, t-test. Scale bar, 5 μm. F THP-1 cells were transfected with Flag-YY1 or Flag-YY1-6R mutant plasmids, with or without 4 μM CHI treatment for 24 h, and detected the expression of METTL3 by qPCR. *P < 0.05; ***P < 0.001, t-test. G The proliferation ability of THP-1 cells transfected with Flag-YY1 or Flag-YY1-6R mutant plasmids, with or without 4 μM CHI treatment, and detected by CCK-8 assay. *P < 0.05; **P < 0.01, one-way ANOVA.

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