Fig. 7: Naa10p regulates cell invasiveness by inducing protease inhibitor expression.

A Protease inhibitor arrays were used to analyze the expression of protease inhibitors in the cell supernatant of KSYE70 transfected with shCtl or shNAA10. B Immunoblotting analysis of stably Naa10p-silencing KYSE70 cells transfected with either Ctl-shRNA or CST6 shRNAs. Quantitative data comparing cell invasion of stably Naa10p-silencing KYSE70 cells transfected with either Ctl-shRNA or CST6 shRNAs. Bars are the mean ± SD of three independent experiments. **P < 0.01 when compared to shCtl group and # P < 0.05 when compared with shNAA10-shCtl cells by two-tailed Student’s t test. C Immunoblotting analysis of stably Naa10p-silencing KYSE70 cells transfected with either Ctl-shRNA or SERPINE1 shRNAs. Quantitative data comparing cell invasion of stably Naa10p-silencing KYSE70 cells transfected with either Ctl-shRNA or SERPINE1 shRNAs. Bars are the mean ± SD of three independent experiments. **P < 0.01 when compared to shCtl group and ## P < 0.01 when compared with shNAA10-shCtl cells by two-tailed Student’s t test. D Immunoblotting analysis of stably Naa10p-silencing KYSE70 cells transfected with either Ctl-shRNA or TIMP2 shRNAs. Quantitative data comparing cell invasion of stably Naa10p-silencing KYSE70 cells transfected with either Ctl-shRNA or TIMP2 shRNAs. Bars are the mean ± SD of three independent experiments. **P < 0.01 when compared to shCtl group and ## P < 0.01 when compared with shNAA10-shCtl cells by two-tailed Student’s t test.