Fig. 3: YTHDC1 promotes cytoplasmic translocation of m6A modified circFNDC3B. | Cell Death & Disease

Fig. 3: YTHDC1 promotes cytoplasmic translocation of m6A modified circFNDC3B.

From: m6A-modified circFNDC3B inhibits colorectal cancer stemness and metastasis via RNF41-dependent ASB6 degradation

Fig. 3

A m6A modification of circFNDC3B was detected by MeRIP-qPCR. B The interaction between biotinylated circFNDC3B and YTHDC1 was verified by RNA pull-down assay. Random probe acted as a negative control. C The interaction between circFNDC3B and YTHDC1 was detected by RIP assay. Normal IgG served as a negative control. D The circFNDC3B level in CRC cells was detected by subcellular fractionation and qRT-PCR. E The subcellular localization of circFNDC3B was detected by RNA FISH. Scale bar, 10 μm; Green, circFNDC3B; Blue, DAPI. **P < 0.01, ***P < 0.001.

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