Fig. 5: OGD/R-ADEX enhances autophagy through AMPK-mTOR signaling. | Cell Death & Disease

Fig. 5: OGD/R-ADEX enhances autophagy through AMPK-mTOR signaling.

From: Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy

Fig. 5: OGD/R-ADEX enhances autophagy through AMPK-mTOR signaling.

a The expressions of p-AMPK/AMPK and p-mTOR/mTOR were determined by western blotting of OGD/R-induced neurons treated with OGD/R astrocytes or OGD/R-ADEXs (n = 5). b The expressions of p-AMPK/AMPK and p-mTOR/mTOR were determined by western blotting of OGD/R-induced neurons treated with OGD/R-ADEXs, Co+GW, or OGD/R-ADEXs+GW (n = 5). c The expressions of p-AMPK/AMPK and p-mTOR/mTOR were determined by western blotting of OGD/R-induced neurons transfected with LV-Nampt or with LV-scramble (n = 5). d The expressions of p-AMPK/AMPK and p-mTOR/mTOR were determined by western blotting of OGD/R-induced neurons transfected with sh-Nampt or sh-NC (n = 5). Data are shown as the mean ± SD of at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Neurons incubated under standard cell culture conditions (Control) were used as negative control; Neurons treated with PBS under OGD/R conditions served as positive control (OGD/R); OGD/R-ADEXs, exosomes isolated from OGD/R astrocytes; Co-culture, OGD/R neurons co-cultured with OGD/R astrocytes; Co+GW4869, OGD/R astrocytes treated with the exosome secretion inhibitor GW4869; OGD/R-ADEXs+GW4869, OGD/R-ADEXs isolated from OGD/R astrocytes pre‐treated with GW4869. ns, not significant.

Back to article page