Fig. 6: OGD/R-ADEXs reduce brain injury and improve neurological function recovery after stroke. | Cell Death & Disease

Fig. 6: OGD/R-ADEXs reduce brain injury and improve neurological function recovery after stroke.

From: Astrocyte-derived exosomal nicotinamide phosphoribosyltransferase (Nampt) ameliorates ischemic stroke injury by targeting AMPK/mTOR signaling to induce autophagy

Fig. 6: OGD/R-ADEXs reduce brain injury and improve neurological function recovery after stroke.

a, b Representative images of cerebral blood flow (CBF) and the percentage change of rCBF compared with baseline during MCAO/R in occlusion and reperfusion. c Representative immunofluorescence images showing the biodistribution of OGD/R-ADEXs within the ischemic cortex. Scale bar: 1 μm. d Neuroprotective effects of OGD/R-ADEXs were evaluated by TTC staining (n = 5). e Quantitation of the infarct volume is shown in (d). f The neurological deficit score was determined in MCAO/R mice treated with OGD/R-ADEXs (n = 5). g, h The sensorimotor function of mice was evaluated using the Corner (g) and rotarod tests (h) in MCAO/R mice treated with OGD/R-ADEXs (n = 5). i The brain water content was measured in MCAO/R mice treated with OGD/R-ADEXs (n = 5). j, k Photomicrograph of mice brain tissue region using hematoxylin and eosin staining of MCAO/R mice brains from mice treated with OGD/R-ADEXs (n = 5). Scale bar: 50 μm. l, m Representative images of Nissl bodies in the ischemic cortex in MCAO/R mice treated with OGD/R-ADEXs (n = 5). Data are shown as the mean ± SD of at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Mice underwent the surgery except that the filament was not inserted into ICA (Sham); Mice was exposed to MCAO followed by administration of PBS (MCAO); OGD/R-ADEXs, exosomes isolated from OGD/R astrocytes.

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