Fig. 9: USP14/MFG-E8 axis involves in CSE-induced ferroptosis in BEAS-2B cells and HBE cells.

A Cells were stimulated with CSE for 24 h and/ or MG132 for 5 h before collection prior to immunoblotting for MFG-E8, GPx4, and SLC7A11. B Empty vectors or USP14-overexpressed plasmids (2 μg/ml) were transfected into cells. Before the cells were collected, the transfected cells were stimulated with CSE for 24 h. USP14, MFG-E8, GPx4, and SLC7A11 were detected by western blot. C Negative control siRNA or USP14 siRNA (50 nM) were transfected into cells. Before the cells were collected, the transfected cells were treated with CSE for 24 h, or combined with MG132 for 5 h. USP14, MFG-E8, GPx4, and SLC7A11 were detected by western blot. D Negative control siRNA or USP14 siRNA were (50 nM) transfected into cells. Before the cells were collected, the transfected cells were treated with CSE for 24 h, or pretreated with rhMFG-E8 for 2 h followed by CSE treatment for 24 h. USP14, MFG-E8, GPx4, and SLC7A11 were detected by western blot. Data are presented as the mean ± SD of three independent experiments. ^*P < 0.05, compared between the marked groups. ^**P < 0.01^, compared between the marked groups. ^***P < 0.001, compared between the marked groups.