Fig. 3: Augmented expression of Col18a1/Endostatin and Serpin E1 by HuD knockdown. | Cell Death & Disease

Fig. 3: Augmented expression of Col18a1/Endostatin and Serpin E1 by HuD knockdown.

From: RNA binding protein HuD mediates the crosstalk between β cells and islet endothelial cells by the regulation of Endostatin and Serpin E1 expression

Fig. 3

A, B After transfection of βTC6 cells with siRNAs, the expression of Col18a1 and Serpin E1 was assessed by RT-qPCR (A) and western blotting analysis (B). Gapdh mRNA was used for normalization. β-actin was used as a loading control. C, D The levels of Col18a1, Endostatin, and Serpin E1 in βTC6 cells (C) or pancreatic tissues (D) were analyzed by immunofluorescence microscopy. The nuclei were stained with DAPI solution. Fluorescent signals between WT and HuD KO mice were quantified using the Image J program (D). Scale bar, 50 μm. Data indicate the mean ± SEM and images are representative of three independent experiments. The statistical significance of the data was analyzed via Student’s t test; *p < 0.05; **p < 0.01; ***p < 0.001.

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