Fig. 7: HuD overexpression decreases the levels of Col18a1 and Serpin E1, while increasing the markers of pericytes and endothelial cells. | Cell Death & Disease

Fig. 7: HuD overexpression decreases the levels of Col18a1 and Serpin E1, while increasing the markers of pericytes and endothelial cells.

From: RNA binding protein HuD mediates the crosstalk between β cells and islet endothelial cells by the regulation of Endostatin and Serpin E1 expression

Fig. 7

A db/db mice were infected with the AAV-HuD and AAV Blank control virus (AAV-Ctrl) (serotype 8) via the tail vein for 4 weeks. RNAs were isolated from the pancreas and relative expression of HuD mRNA was assessed by RT-qPCR. Gapdh mRNA was used for normalization. B, C Relative levels of Col18a1, Serpin E1, NG2, and PECAM-1 in the pancreatic tissues were analyzed by immunofluorescence microscopy. The nuclei were stained with DAPI solution. Fluorescent signals between AAV-Ctrl and AAV-HuD groups were quantified using the Image J program. Data indicate the mean ± SEM and images are representative of three independent experiments. Scale bar, 50 μm. The statistical significance of the data was analyzed via Student’s t test; *p < 0.05; **p < 0.01.

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