Fig. 5: Ca-074Me and GSDMD have non-redundant roles on IL-1β release in response to flagellin. | Cell Death & Disease

Fig. 5: Ca-074Me and GSDMD have non-redundant roles on IL-1β release in response to flagellin.

From: Lysosomal cathepsins act in concert with Gasdermin-D during NAIP/NLRC4-dependent IL-1β secretion

Fig. 5

Starch-elicited peritoneal macrophages (PMs) isolated from C57BL/6 WT or Gsdmd−/− mice were pretreated with the cathepsin B inhibitor Ca-074Me, primed with LPS and incubated with ultrapure flagellin extracted from Salmonella typhimurium inserted into DOTAP (FliDot). A IL-1β secretion was evaluated in the culture supernatants by ELISA after 3 h of stimulation. Data represent two experiments performed in technical triplicates ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 (Three-way ANOVA). B GSDMD cleavage was determined in the culture supernatants by ELISA after 3 h and 6 h of stimulation. Data represent two (FliDot 6 h) or three (FliDot 3 h) independent experiments performed in technical triplicates ± SD.

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