Fig. 2: Elevated HN1L enhances ESCC metastasis. | Cell Death & Disease

Fig. 2: Elevated HN1L enhances ESCC metastasis.

From: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma

Fig. 2

A HN1L expression in normal esophageal epithelial cell NE1 and ESCC cell lines were analyzed by western blotting. B Western blotting was used to confirm the exogenous overexpression of HN1L in KYSE30 cells. C Silence of HN1L in KYSE150 cell with shRNA was confirmed by western blotting analysis. D Transwell assay using KYSE30-Vector and KYSE30-HN1L cells. E Transwell assay with KYSE150-scramble and KYSE150-shHN1L cells. F Lung metastasis of KYSE30-Vector and KYSE30-HN1L cells were tested by tail vein injection in nude mice and H&E staining of lung tissues was performed. Scale bar, 250 μm. G The rate of mice with lung metastasis was counted. H H&E staining of lung tissues from nude mice after tail vein injection of KYSE150-scramble and KYSE150-shHN1L cells. Scale bar, 250 μm. I The rate of mice with lung metastasis was counted. In all panels, data are presented as the mean ± SD; two-sided Student’s t test; **P < 0.01, ***P < 0.001.

Back to article page