Fig. 5: HN1L drives ESCC cell growth and migration by up-regulating PLK1. | Cell Death & Disease

Fig. 5: HN1L drives ESCC cell growth and migration by up-regulating PLK1.

From: HN1L/AP-2γ/PLK1 signaling drives tumor progression and chemotherapy resistance in esophageal squamous cell carcinoma

Fig. 5

A The heat map showed the expressions of Rho GTPase effectors after HN1L overexpression. B Western blotting was used to confirm the expression of PLK1 after HN1L overexpression or silence. C Double IF staining showed the co-expression of HN1L and PLK1 in KYSE30 cells. Scale bar, 20 μm. D BrdU incorporation assay showed the cell proliferation rate of KYSE30-Vector and KYSE30-HN1L after PLK1 silence. Scale bar, 20 μm. E Transwell migration assay was performed to test the cell migration ability of KYSE30-Vector and KYSE30-HN1L after PLK1 knockdown. F Lung metastasis experiment showed that overexpression of HN1L enhanced the metastatic ability of KYSE30 cells, and PLK1 knockdown reduced the metastasis rate of KYSE30-HN1L cells in the lung. Scale bar, 250 μm. In D, E, data are presented as the mean ± SD; two-sided Student’s t test; *P < 0.05, **P < 0.01, ns: no significant difference.

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