Fig. 2: Migration- and EMT-related markers were differentially expressed between the trisomic and euploid cells.

a qPCR assay for epithelial markers (ECAD, CLDN7) in day 7 cells. b Western blotting analysis for the protein expression level of ECAD in day 7 cells. c qPCR assay for premigratory markers (MSX2, DLX5, SOX9) in day 7 cells. d qPCR assay for postmigratory markers (p75, SOX10, SNAI2) in day 7 cells. Data are presented as the mean ± SD of three independent experiments. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, two-tailed unpaired Student’s t test.