Fig. 2: Hepatocytes Tsc1 ablation protects liver against Con-A induced hepatocyte injury. | Cell Death & Disease

Fig. 2: Hepatocytes Tsc1 ablation protects liver against Con-A induced hepatocyte injury.

From: Mechanistic target of rapamycin complex 1 orchestrates the interplay between hepatocytes and Kupffer cells to determine the outcome of immune-mediated hepatitis

Fig. 2

a The strategy for hepatocytes Tsc1 deletion in Alb-Cre+/−, Tsc1fl/fl mice with Con-A administration. b The ALT levels in serum, n = 6. c Representative HE and TUNEL- stained mouse livers. Scale bar = 200 μm. d Representative co-immunofluorescent staining images for F4/80 with p-S6 (white arrows). Scale bar = 100 μm. e Quantitative determination of F4/80+ cells among groups as indicated, n = 3. f Left, representative co-immunofluorescent staining images for F4/80 with CCP3 (white arrows). Scale bar = 50 μm. Right, quantification, n = 4. g The co-culture system. h Western blot assay showing the abundance of TSC1 in primary hepatocytes. i Left, representative pi-staining images for primary KCs were cultured with conditioned media (CM) from Tsc1-/- hepatocytes for 48 h. Scale bar = 100 μm. Right, quantitative determination of Pi+ KCs among groups as indicated, n = 5. j Left, representative Pi-staining images for primary KCs cultured with CM treated with RNase or 99 °C boiled for 48 h. Scale bar = 100 μm. Right, quantitative determination of Pi+ KCs among groups as indicated, n = 3. k Left, representative Pi-staining images for primary KCs cultured with exosomes or exosomes free-CM for 48 h, respectively. Scale bar = 100 μm. Right, quantification, n = 3. *p < 0.05.

Back to article page