Fig. 3: In vivo investigation of the interplay between α-syn and Hb. | Cell Death & Disease

Fig. 3: In vivo investigation of the interplay between α-syn and Hb.

From: Neuronal haemoglobin induces loss of dopaminergic neurons in mouse Substantia nigra, cognitive deficits and cleavage of endogenous α-synuclein

Fig. 3

Representative immunoblotting with SYN-1 (a upper membrane) and Hb (a lower membrane) antibodies of the SN tissue of WT mice (n = 3) subjected to Hb immunoprecipitation. Representative immunoblotting with SYN-1 (b upper membrane) and Hb (b lower membrane) antibodies of the SN tissue of CTRL and Hb mice (n = 5) subjected to Hb immunoprecipitation. Hb staining (a and b) has been used as internal control. Schematic representation of the domain structure of human α-syn protein and epitopes of anti-α-syn antibodies used for the study (c). FL-α-syn (17 KDa), ΔC-α-syn (11 KDa) expression in SN of Hb and CTRL mice (n = 8) at 10 months post-injection of (d left panel) was assessed by western blot. Band intensity was quantified (d right panel). I, input; (-), control resin with no antibody; IP, immunoprecipitated samples using anti-Hb antibody (see “Materials and methods”). Data represent means ± SEM. Statistical analysis was performed with unpaired t-test with Welch’s correction. *p ≤ 0.05.

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