Fig. 4: TNC promoted fibroblasts proliferation. | Cell Death & Disease

Fig. 4: TNC promoted fibroblasts proliferation.

From: Matrix protein Tenascin-C promotes kidney fibrosis via STAT3 activation in response to tubular injury

Fig. 4

In vivo, EdU positive cells were markedly increased and predominantly located in the renal interstitium after UUO, and TNC deletion significantly reduced the number of EdU-positive cells in the obstructed kidney compared with wild-type mouse (A, n = 4 mice, 3 slides for each mouse, p < 0.05). To further examine the effect of TNC on cell proliferation, TNC-expressing cells (TNC-Cell) were obtained by sorting the tdTomato-positive cells in UUO kidneys, and then immortalized by transfecting SV40 T lentivirus (B). Exogenous TNC dose dependently increased the cell number of TNC-Cell, NIH3T3 and NRK49F in vitro, assessed by CCK8 kit (C–E, p < 0.01). Exogenous TNC also markedly increased EdU incorporation in NRK49F cells, consistent with promoting cell proliferation (F, p < 0.001).

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